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IMEter v2.0

Welcome to the new improved version of the IMEter (v2.0). The IMEter calculates if an intron is likely to enhance gene expression. The higher the IMEter the score, the more likely the intron is expected to enhance expression. The IMEter has been trained using Arabidopsis thaliana intron data, but we believe it is useful for predicting the expression enhancing ability of introns from a wide variety of plant species. The older version of the IMEter is still available.

To assess the IMEter 2.0 score of your intron, here are the IMEter v2.0 scores at every 5% interval from 85,375 high-confidence Arabidopsis thalian introns (from TAIR10 annotations). E.g. 50% of all Arabidopsis introns have an IMEter v2.0 score that is less than, or equal to 4.50:

5% introns <= 0.00
10% introns <= 0.94
15% introns <= 1.27
20% introns <= 1.69
25% introns <= 2.20
30% introns <= 2.64
35% introns <= 3.08
40% introns <= 3.53
45% introns <= 4.00
50% introns <= 4.50
55% introns <= 5.04
60% introns <= 5.64
65% introns <= 6.33
70% introns <= 7.11
75% introns <= 8.05
80% introns <= 9.25
85% introns <= 10.88
90% introns <= 13.49
95% introns <= 19.00
100% introns <= 77.90

Introns that moderately enhance expression tend to have IMEter v2.0 scores above 10 and introns that strongly enhance expression tend to have scores above 20. Though there are always exceptions to this.

Reference: Parra, G., Bradnam, K., Rose, A. and Korf, I. Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants Nucleic Acids Research, 2011

IMEter v2.0

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